esiSCRIBE is a very versatile T7 RNA-polymerase-based in vitro transcription kit, suitable to produce single- or double-stranded RNA.
Applications of the produced single-stranded (ss)RNA include guide RNA for CRISPR/Cas9 experiments and hybridization probes for FISH (fluorescence in situ hybridization), RNA SELEX (systematic evolution of ligands by exponential enrichment), Northern blots etc.
The most popular application of double-stranded (ds)RNA transcribed with esiSCRIBE is RNA interference (RNAi).
The reliability of the esiSCRIBE kit and quality of the resulting dsRNAs was validated in a large-scale RNAi screen, which applied dsRNAs obtained with the esiSCRIBE kit (Kittler R. et al., Genome-wide resources of endoribonuclease-prepared short interfering RNAs for specific loss-of-function studies, Nature Methods, 2007, 4, 337-344.)
esiSCRIBE is easy to handle and easy to use: No special equipment is required. A detailed protocol and all necessary reagents are included.
esiSCRIBE – In Vitro Transcription Kit for 179.00 EUR
High Yield: One 10-µl reaction delivers > 25 µg of RNA (100 reactions per kit).
Template: The DNA template for the RNA production must contain a T7 promoter sequence.
Components:10x Reaction Buffer, RNase-free water, NTP mix (ATP, CTP, GTP, UTP; 25 mM each), T7 RNA polymerase and a positive control.
For further details, please see the application manual.